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CMC Correlative Microscopy Coverslips
affordable polyester film cover slips with practical patterns



Introduction

The CMC Correlative Microscopy Coverslips are made using a polyester based film with unique reticle patterns. They are specifically designed to allow indentification and location of particular areas of interest with brightfield or fluorescence microscopes and then processing for electron microscopy.
The material of the CMC correlative microscopy coverslips is compatible with cell culture growth and exhibit good adhesion for cell cultures.

Features and benefits of the CMC correlative microscopy coverslips:

  • good optical quality in brightfield and fluorescence microscopy
  • excellent transparency
  • use temperature range -196°C to +100°C
  • rigid – does not float in the middle of culture
  • resistant to normal chemicals used in electron microscopy
  • no oxygen retention; compatible with LR white resin
  • simple sterilisation using alcohol or UV
  • easy to handle and easy to cut with a knife or punch
  • choice of three different field proven patterns
  • low cost


CMC Correlative Microscopy Coverslips specifications:

Product

CMC 34A

CMC 35

CMC 71

Product #

51-002834

51-002835

51-002871

Pattern size

5 ea 1x1mm

10x10mm

10x10mm

Divisions

0.1x0.1mm

1x1mm

0.5x0.5mm

Thickness

0.18mm

Material

Polyester

Coverslip size

22x22mm

The CMC correlative microscopy coverslips are not designed to replace the optically superior quartz or glass coverslips; the film itself has a structure which can be visible in certain illumination conditions. The CMC correlative coverslips are designed for correlative microscopy using different imaging techniques. An initial general analysis is performed using light microscopy and then the sample is further processed for SEM or TEM analysis. The key advantage is that the film is easily cut and punched to allow EM processing.

How to use the CMC correlative microscopy coverslips:

  1. Sterilise the CMC correlative coverslip with alcohol, dry and add culture
  2. Ensure that the grid of the correlative coverslip is positioned correctly and that the text is readable
  3. Record images needed and note the co-ordinates of the region of interest
  4. Fix, dehydrate and embed with resin for TEM
  5. Place a BEEM capsule, filled with reson onto the coverslip over the selected region of interest
  6. Cure resin, detach the correlative coverslip; the footprint of the grid in the resin shows the selected location, trim the block in the selected area and make cuts using an ultramicrotome



Ordering Information forCMC Correlative Microscopy Coverslips

*Prices are without tax. Tax might be added in cart.


    # 51-002834   CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisions

CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisionsCMC34A - 10 x 10 grids of 0.1mm squares at 5 positions. Indexed 1-10 along the top and A-J down the side. Five positions of 1x1mm each are indexed A-E. Total area 5 x 1mm2

CMC34A correlative coverslips 5 x 1x1mm with 0.1mm divisions
Product # Unit Price* Add to Quote / Cart
51-002834 pkg/25 €78,50
Qty:



    # 51-002835   CMC35 correlative coverslips 10x10mm with 1mm divisions

CMC35 correlative coverslips 10x10mm with 1mm divisionsCMC35 - 10 x 10 grid of 1mm squares. Each individually indexed 0-99. Total area 100mm2.

CMC35 correlative coverslips 10x10mm with 1mm divisions
Product # Unit Price* Add to Quote / Cart
51-002835 pkg/25 €64,50
Qty:



    # 51-002871   CMC71 correlative coverslips 10x10mm with 0.5mm divisions

CMC71 correlative coverslips 10x10mm with 0.5mm divisionsCMC71 - 20 x 20 grid of 0.5mm squares. Indexed 1-20 along the top, A-T down the side and repeated on centre cross. Total area 100mm2.

CMC71 correlative coverslips 10x10mm with 0.5mm divisions
Product # Unit Price* Add to Quote / Cart
51-002871 pkg/25 €64,50
Qty:


 




SEM Supplies

TEM Supplies

Calibration

Sample Preparation

AFM / SPM

  SEM Sample Stubs
  Sample Stub Adapters
  SEM Stage Adapters
  SEM Sample Holders
  SEM Preparation Stands
  Filaments/Cathodes
  Silicon Finder Grid
  Gatan 3View Pins
  FEI Volumescope Pins
  SEM Stub Storage Boxes
  Phenom Supplies
  JEOL NeoScope Supplies
  Hitachi TM series Supplies
  Field & lab sampler kits

Instruments


  Acoustic Enclosures
  Critical Point Dryers
  Cressington SEM Coaters
  HI-Res IR Chamberscope
  TEM Sample Prep
  TEM Grids
  TEM Support Films
  Graphene Films
  Grid Storage Boxes
  Cryo Grid Box
  TEM sample staining
  Filaments/Cathodes
  Cryo Pin for U-M
  Eyelash manipulators

FIB Supplies


  FIB Lift-out grids
  FIB low profile stubs
  FIB grid holders
  FIB pre-tilt holders
  FIB Grid Boxes
  SEM / FIB Magn. Calib.
  SEM Res. Test Spec.
  EDS / WDS Calibration
  TEM Calibration
  AFM / SPM Calibration
  LM Magn. Calibration

Vacuum Supplies


  Bullseye Vacuum Gauges
  D215V Vacuum Controller
  D801W Vacuum Gauges
  Quick-check Gauges
  KF/NW Vacuum Parts
  Vacuum oil and grease
  Vacuum sample storage
  Evaporation baskets
  Quartz QCM Crystals
  Sputter Targets
  Carbon Rods & Fibers
  Quartz QCM Crystals
  Supports & Substrates
  SEM Preparation Stands
  Tweezers
  Probes & Picks
  Cryo Jug & Container
  Applicators & Swabs
  Plastic Transfer Pipettes
  Cutting Tools / Scissors
  Conductive Paint/Cement
  Conductive Tapes &Tabs
  Non-conductive Adhesives
  Stainless Steel Mesh
  Sample Storage
  Tools
  Preparation mats & tiles
  Cleaning / Gloves
  Diamond Polishing Paste
  AFM/SPM Discs
  AFM/SPM Holders
  AFM/SPM Disc Storage
  Cantilever Tweezers
  AFM/SPM Discs Tweezers
  Mica Discs
  HOPG Substrates


LM


  Correlative Cover Slips
  Glass Microscope Slides
  Glass Cover Slips
  Quartz Microscope Slides
  Quartz Cover Slips
  Black metal slides
  Slide storage boxes
  Calibration
  Plastic Transfer Pipettes