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UranyLess TEM staining alternative for Uranyl Acetate
fast-acting, non-radioactive lanthanide staining mix



Unranyless introduction


Introduction

UranyLess is an efficient, ready to use staining substitute for uranyl acetate delivering similar results. With Uranyless there is an real alternative  to uranyl acetate, without the problems with restrictions, toxicity and waste elimination procedures associated with uranyl acetate.

      UranyLess applied
      Staining protocol:
  1. Place a droplet of UranyLess on parafilm or a hydrophobic slide
  2. Place the TEM grid on the UranyLess droplet for 1-2 minutes
  3. Blot the grid with filter paper
  4. Then wash the grid in distilled water
  5. Let the grid with sample dry

UranyLess is a proprietary mix of multiple lanthanides with a great affinity for biological material and comes as a ready to use aqueous solution. It has been tested on many biological tissue, both animal and plant. The UranyLess staining solution is ideal for ultra-fine sections and negative staining.  UranyLess is an efficient, non-radioactive lanthanide staining mix. After only one minute of contact, the sections or deposits are stained to provide contrast for subsequent TEM imaging. If needed, lead citrate is recommended to enhance contrast; classic staining protocol after Reynolds (1963).

UranyLess is supplied in an Airless bottle with a content of 30ml. The Airless bottle is filled without air and delivers droplets of the solution through a push-pump system. This eliminates CO2 contamination.  With 30ml you can stain approximately 1500 TEM grids. The UranyLess solution in the Airless bottle has a long shelf life, eliminates CO2 contaminations and avoids waste by delivery in droplets. The pH level of UranyLess is 6.8 to 7. Alternative packaging size is 200ml for the Leica EM Stain automatic stainer.

Lead citrate. Although UranyLess has a strong contasting effect, it is recommended to use lead citrate to enhance the contrast following the classic staining protocol according to Reynolds. We offer a ready to use 3% lead citrate solution in an Airless bottle which is filled w/o air and delivers the lead citrate droplets through a push-pump system thus eliminating CO2 contamination.


Videos: (In French, but easy to follow without any knowledge of French)
Classic staining protocol of thin section with UranyLess  Staining with UranyLess and contrast enhancement with lead citrate


Technical Support Bulletin: UranyLess staining protocols
FAQ: FAQ


Ordering information

*Prices are without tax. Tax might be added in cart.

UranyLess, uranium-free, aqueous staining solution, 30ml Airless bottle UranyLess, uranium-free, aqueous staining solution, 30ml Airless bottle

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Product # Unit Price* Add to Quote / Cart
29-005030 each €28,00
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Extra info 29-005030 :
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UranyLess, uranium-free, aqueous staining solution for Leica EM stain, 200ml bottle UranyLess, uranium-free, aqueous staining solution for Leica EM stain, 200ml bottle
Product # Unit Price* Add to Quote / Cart
29-005035 each €166,00
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Lead Citrate 3%, contrast enhancement solution, 30ml Airless bottle Lead Citrate 3%, contrast enhancement solution, 30ml Airless bottle

Hazardous Symbol Explantion

hazard fee symbol = Hazard Shipping Fee
Cg = Suspected Carcinogen
F = Flammable
I = Irritant
S = Sensitizer
T = Toxic
Product # Unit Price* Add to Quote / Cart
29-005040 each €28,00
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Extra info 29-005040 :
    SDSpdf download    



FAQ – Frequently asked questions about UranyLess


Q: What is Uranyless made from?
A Uranyless is a mix of lanthanides in an aqueous solution eample of classic staining with UranlyLess

Q: What is the shelf life of UranyLess?
A: Unlimited; with a 30ml bottle it should cover a year of average use

Q: What are the storage conditions for UranyLess?
A: Store at room temperature; it is not light sensitive

Q: How can Uranyless be used?
A: It is ready for use; no dilution needed – see Technical Support Bulletin

Q: What is the pH of UranyLess?
A: It is 6.8-7, different to uranyl acetate which has a pH of 4

Q: How to stain with UranyLess?
A: Place your grid on a droplet of UranyLess, wait a minute. Dry and then contrast with lead citrate according the Reynolds protocol.

Q: Is it the same protocol for different  kind of tissue?
A: Yes, it is the same for animal, plant, marine tissue; UtanyLess and contrast enhancement with lead citrate

Q: Is it compatible with different type of resins?
A: Yes, it works with different embedding resins (Epon, Spurr, Araldite….)

Q: Can UranyLess be used for negative staining?
A: Yes, see Technical Support Bulletin

Q: Can it be used for block staining?
A: Test are in progress

Q: Can UranyLess be used at cryo applications?example of staining
A: No; it is an aquous solution which will freeze. Current developments are other Uranyless solution in alcohol and acetone.

Q: Can UranyLess be used on the Leica EM Stain?
A: Yes, the 200ml packaging is compatible with the Leica EM Stain

Q: How do dispose of UranyLess waste?
A: UranyLess contains lanthanides (heavy elements) and need to be disposed according to rules for heavy elements.

Q: How is UranyLess packaged?
A: Standard packaging is a 30ml Airless bottle to avoid CO2 contamination, alternatively it is available in a 200ml square glass bottle

Q: How does the Airless bottle operate?
A: It is a bottle which doesn’t let air in (eliminates CO2 contamination). Simply press the top of the bottle to get a drop delivered. When you release the pump actuator lifts the head up.

Q: What are the advantages of the Airless bottle?
A: It doesn’t let air in to eliminate CO2 contamination. It delivers drop by drop, ready to use and it reduces waste.  With the Airless bottle, the product has a longer shelf life.



SEM Supplies

TEM Supplies

Calibration

Sample Preparation

AFM / SPM

  SEM Sample Stubs
  Sample Stub Adapters
  SEM Stage Adapters
  SEM Sample Holders
  SEM Preparation Stands
  Filaments/Cathodes
  Silicon Finder Grid
  Gatan 3View Pins
  FEI Volumescope Pins
  SEM Stub Storage Boxes
  Phenom Supplies
  JEOL NeoScope Supplies
  Hitachi TM series Supplies
  Field & lab sampler kits

Instruments


  Acoustic Enclosures
  Critical Point Dryers
  Cressington SEM Coaters
  HI-Res IR Chamberscope
  TEM Sample Prep
  TEM Grids
  TEM Support Films
  Graphene Films
  Grid Storage Boxes
  Cryo Grid Box
  TEM sample staining
  Filaments/Cathodes
  Cryo Pin for U-M
  Eyelash manipulators

FIB Supplies


  FIB Lift-out grids
  FIB low profile stubs
  FIB grid holders
  FIB pre-tilt holders
  FIB Grid Boxes
  SEM / FIB Magn. Calib.
  SEM Res. Test Spec.
  EDS / WDS Calibration
  TEM Calibration
  AFM / SPM Calibration
  LM Magn. Calibration

Vacuum Supplies


  Bullseye Vacuum Gauges
  D215V Vacuum Controller
  D801W Vacuum Gauges
  Quick-check Gauges
  KF/NW Vacuum Parts
  Vacuum oil and grease
  Vacuum sample storage
  Evaporation baskets
  Quartz QCM Crystals
  Sputter Targets
  Carbon Rods & Fibers
  Quartz QCM Crystals
  Supports & Substrates
  SEM Preparation Stands
  Tweezers
  Probes & Picks
  Cryo Jug & Container
  Applicators & Swabs
  Plastic Transfer Pipettes
  Cutting Tools / Scissors
  Conductive Paint/Cement
  Conductive Tapes &Tabs
  Non-conductive Adhesives
  Stainless Steel Mesh
  Sample Storage
  Tools
  Preparation mats & tiles
  Cleaning / Gloves
  Diamond Polishing Paste
  AFM/SPM Discs
  AFM/SPM Holders
  AFM/SPM Disc Storage
  Cantilever Tweezers
  AFM/SPM Discs Tweezers
  Mica Discs
  HOPG Substrates


LM


  Correlative Cover Slips
  Glass Microscope Slides
  Glass Cover Slips
  Quartz Microscope Slides
  Quartz Cover Slips
  Black metal slides
  Slide storage boxes
  Calibration
  Plastic Transfer Pipettes